The present study describes the effects of periodically amputated rat incisor teeth upon the weight of submaxillary gland, the distribution and content of phosphrous compound, ie., acid soluble phosphorus, lipid phosphorus, nucleic acid phosphorus, and protein phosphorus, which are all important parameters on the cellular component changes in submaxillary gland, and evaluates the relationship between the surgical injury and general metabolism by means of serum protein bound hexose and fucose concentration.
The male rats were divided into two groups:
1) Amputated group : rats with repeated amputation on the upper and lower four incisors.
2) Control group : rats with no incisor amputation.
The incisor amputation was performed under ´light ether anesthesia, with bone cutting forcep two or three milimeter above the gingival border, every two days during two weeks.
At the end of experiment, two days after the last amputation, the rats were decaptalized, the blood was obtained, . the submaxillary glands were removed, weighed and homogenized.
The phosphorus compound was fractionated by Schneider´s method, the phosphorus content of each fraction was determined by Fiske-SubbaRow method. Protein bound hexose with orcinol-H₂SO₄ reagent and protein bound fucose with cysteine reagent were quantitated.
The results were obtained as follows.
1. The weight of submaxillary gland after the incisor amputation was increased about two times than control group.
2. The distribution rates of phosphorus compound in each fraction were almost similar to those of control group.
3. The concentrations of serum nrotein bound hexose and fucose after incisor amputation increased by 12.6%, 46.7%, respectively.
4. By increasing of the weight of submaxillary gland, the contents of acid soluble phosphorus, lipid phosphorus, nucleic acid phosphorus, protein phosphorus, and total phosphorus increased by 85.6%, 96.6%, 76.9%, 85.5% and 85.5%, respectively.
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